| ABAmes Branch | Ames, IA | Beverage and Tobacco Product Manufacturing312111 | 20–99 | ◐ LIKELY ACTIVE |
| ARAya Ranch, L.c. | Ames, IA | Beverage and Tobacco Product Manufacturing312130 | — | ◐ LIKELY ACTIVE |
| BABASF Agricultural Solutions US LLC | Ames, IA | Chemical Manufacturing325130 | 20–99 | ◐ LIKELY ACTIVE |
| BHBig Howdys Meadery | Boone, IA | Beverage and Tobacco Product Manufacturing312130 | — | ◐ LIKELY ACTIVE |
| BLBiova LLC | Ames, IA | Food Manufacturing311999 | 1–19 | ◐ LIKELY ACTIVE |
| BCBluff Creek Distillery, L.l.c. | Boone, IA | Beverage and Tobacco Product Manufacturing312140 | — | ◐ LIKELY ACTIVE |
| CPCoherent Photon Imaging, LLC | Ames, IA | Computer and Electronic Product Manufacturing334516 | — | ◐ LIKELY ACTIVE |
| DDSM-Firmenich | Ames, IA | Food Manufacturing311111 | 20–99 | ◐ LIKELY ACTIVE |
| GWGary W Clem, Inc. dba ALMACO | Nevada, IA | Machinery Manufacturing333111 | 100–499 | ◐ LIKELY ACTIVE |
| GFGeneral Financial Supply | Nevada, IA | Printing and Related Support Activities323111 | 20–99 | ◐ LIKELY ACTIVE |
| IIowa | Nevada, IA | Paper Manufacturing322230 | 20–99 | ◐ LIKELY ACTIVE |
| ISIowa State University Meat Laboratory | Ames, IA | Food Manufacturing311611 | — | ◐ LIKELY ACTIVE |
| LMLester Mechanical LLC | Nevada, IA | Machinery Manufacturing333415 | — | ◐ LIKELY ACTIVE |
| LSLs Story City | Story City, IA | Wood Product Manufacturing321215 | 20–99 | ◐ LIKELY ACTIVE |
| MAMerck Ames, IA Facility | Ames, IA | Chemical Manufacturing325414 | 20–99 | ◐ LIKELY ACTIVE |
| MMMid-States Material Handling & Fabrication, Inc. | Nevada, IA | Fabricated Metal Product Manufacturing332312 | 20–99 | ◐ LIKELY ACTIVE |
| NnameA | Huxley, IA | Chemical Manufacturing325412 | 100–499 | ◐ LIKELY ACTIVE |
| OVOttawa Vineyards, L.l.c. | Madrid, IA | Beverage and Tobacco Product Manufacturing312130 | — | ◐ LIKELY ACTIVE |
| PIParagon International | Nevada, IA | Machinery Manufacturing333241 | 20–99 | ◐ LIKELY ACTIVE |
| PBPDM Boone 2419 Industrial | Boone, IA | Food Manufacturing311225 | 20–99 | ◐ LIKELY ACTIVE |
| PCPolecat Creek Winery | Boone, IA | Beverage and Tobacco Product Manufacturing312130 | — | ◐ LIKELY ACTIVE |
| PVPrairie Vineyards, L.c. | Story City, IA | Beverage and Tobacco Product Manufacturing312130 | — | ◐ LIKELY ACTIVE |
| PPrintgraphics | Nevada, IA | Printing and Related Support Activities323111 | 1–19 | ◐ LIKELY ACTIVE |
| PBProliant Biologicals - Boone | Boone, IA | Chemical Manufacturing325414 | 20–99 | ◐ LIKELY ACTIVE |
| QAQuill Avenue | Boone, IA | Chemical Manufacturing325414 | 20–99 | ◐ LIKELY ACTIVE |
| S2Salin 247, INC. | Boone, IA | Machinery Manufacturing333111 | — | ◐ LIKELY ACTIVE |
| STShivvers Technology & Innovation Group | Ames, IA | Machinery Manufacturing333111 | 1–19 | ◐ LIKELY ACTIVE |
| SCStory City | Story City, IA | Transportation Equipment Manufacturing336212 | 100–499 | ◐ LIKELY ACTIVE |
| SASukup Ames Satellite Office | Ames, IA | Machinery Manufacturing333111 | 20–99 | ◐ LIKELY ACTIVE |
| TRTalus Renewables - Boone, IA | Boone, IA | Chemical Manufacturing325311 | 1–19 | ◐ LIKELY ACTIVE |
| TNTech Network Supply LLC | Ames, IA | Computer and Electronic Product Manufacturing334112 | — | ◐ LIKELY ACTIVE |
| VNVerbio - Nevada | Nevada, IA | Chemical Manufacturing325193 | 20–99 | ◐ LIKELY ACTIVE |
| WBWilderness Brew Co. | Nevada, IA | Beverage and Tobacco Product Manufacturing312130 | — | ◐ LIKELY ACTIVE |
| SCStory City Locker | Story City, IA | Food Manufacturing311611 | — | ◇ UNVERIFIED |
| APAir Products and Chemicals, INC. Co2 Recovery Plant | Nevada, IA | Chemical Manufacturing325120 | — | ◇ UNVERIFIED |
| AIApc INC. | Boone, IA | Food Manufacturing311119 | — | ◇ UNVERIFIED |
| BLB5Branding LLC | Ames, IA | Printing and Related Support Activities323113 | — | ◇ UNVERIFIED |
| BDBlackbird Design and Print LLC | Nevada, IA | Printing and Related Support Activities323111 | — | ◇ UNVERIFIED |
| CCopyworks | Ames, IA | Printing and Related Support Activities323111 | — | ◇ UNVERIFIED |
| CSCustom Steel Service | Ames, IA | Fabricated Metal Product Manufacturing332312 | — | ◇ UNVERIFIED |
| DEDCT Enterprises, LLC | Ames, IA | Printing and Related Support Activities323111 | — | ◇ UNVERIFIED |
| DLDesh LLC | Ames, IA | Food Manufacturing311811 | — | ◇ UNVERIFIED |
| DNDsm Nutritional Products | Ames, IA | Food Manufacturing311119 | — | ◇ UNVERIFIED |
| HEHammond Enterprises LLC | Boone, IA | Food Manufacturing311811 | — | ◇ UNVERIFIED |
| HMHawkeye Molding Company Inc | Roland, IA | Plastics and Rubber Products Manufacturing326199 | — | ◇ UNVERIFIED |
| ILInnovative Lighting, INC. | Roland, IA | Transportation Equipment Manufacturing336321 | — | ◇ UNVERIFIED |
| IIIntervet INC Dba Merck Animal Health Building 1 | Ames, IA | Chemical Manufacturing325414 | — | ◇ UNVERIFIED |
| IIIntervet INC Dba Merck Animal Health - Building 4 | Ames, IA | Chemical Manufacturing325414 | — | ◇ UNVERIFIED |
| IIIntervet INC Dba Merck Animal Health - Research Barn | Ames, IA | Chemical Manufacturing325414 | — | ◇ UNVERIFIED |
| JMJa Max Machine CO. INC. | Roland, IA | Transportation Equipment Manufacturing336311 | — | ◇ UNVERIFIED |