| ACAfinitas- Columbus Location | Columbus, OH | Machinery Manufacturing333249 | 20–99 | ◐ LIKELY ACTIVE |
| AAAGC Automotive Americas Replacement Glass (ARG) - Grove City | Grove City, OH | Nonmetallic Mineral Product Manufacturing327215 | 1–19 | ◐ LIKELY ACTIVE |
| ANAkzo Nobel Coatings Inc (2) | Columbus, OH | Chemical Manufacturing325510 | 100–499 | ◐ LIKELY ACTIVE |
| ALAlleguard - Lancaster | Lancaster, OH | Plastics and Rubber Products Manufacturing326140 | 20–99 | ◐ LIKELY ACTIVE |
Altium Packaging - Columbus | Columbus, OH | Plastics and Rubber Products Manufacturing326160 | 20–99 | ◐ LIKELY ACTIVE |
| ARAmcor Rigid Packaging New Albany | New Albany, OH | Plastics and Rubber Products Manufacturing326160 | 100–499 | ◐ LIKELY ACTIVE |
| AHAmerican Health Packaging | Columbus, OH | — | — | ◐ LIKELY ACTIVE |
| AHAmerican Home Health Care INC | Columbus, OH | Miscellaneous Manufacturing339112 | — | ◐ LIKELY ACTIVE |
| ALAmericold Logistics, LLC | Columbus, OH | — | — | ◐ LIKELY ACTIVE |
| AAm-hub | Worthington, OH | Machinery Manufacturing333998 | 100–499 | ◐ LIKELY ACTIVE |
| ATAnalytical Testing Corp, LLC | Columbus, OH | Computer and Electronic Product Manufacturing334516 | — | ◐ LIKELY ACTIVE |
| AHAnchor Hocking | Lancaster, OH | Nonmetallic Mineral Product Manufacturing327212 | 500+ | ◐ LIKELY ACTIVE |
| ACAN Cleveland Ave Div TC | Columbus, OH | Food Manufacturing311514 | 20–99 | ◐ LIKELY ACTIVE |
| ACAN Columbus Plant | Columbus, OH | Food Manufacturing311514 | 500+ | ◐ LIKELY ACTIVE |
| AIAncora Imparo, LLC | Columbus, OH | Fabricated Metal Product Manufacturing332721 | — | ◐ LIKELY ACTIVE |
| ACAnderson Concrete Corp. Whole Company | Columbus, OH | Nonmetallic Mineral Product Manufacturing327320 | 100–499 | ◐ LIKELY ACTIVE |
| AEAN Easton Square | Columbus, OH | Food Manufacturing311514 | 500+ | ◐ LIKELY ACTIVE |
| ACAnomatic Corporation- Blacklick | Blacklick, OH | Plastics and Rubber Products Manufacturing326199 | 20–99 | ◐ LIKELY ACTIVE |
| ACAnomatic Corporation- New Albany | New Albany, OH | Fabricated Metal Product Manufacturing332813 | 100–499 | ◐ LIKELY ACTIVE |
| ALAnro Logistics, INC | Westerville, OH | Fabricated Metal Product Manufacturing332322 | — | ◐ LIKELY ACTIVE |
| ARAN Ross Park | Columbus, OH | Food Manufacturing311514 | 500+ | ◐ LIKELY ACTIVE |
| ATAnthony Thomas Candy Co., Inc. | Columbus, OH | Food Manufacturing311352 | 100–499 | ◐ LIKELY ACTIVE |
| AOA. O. Smith Groveport | Groveport, OH | Machinery Manufacturing333318 | 100–499 | ◐ LIKELY ACTIVE |
| AMAppian Manufacturing Corp | Columbus, OH | Fabricated Metal Product Manufacturing332996 | 20–99 | ◐ LIKELY ACTIVE |
| AMArdent Mills Columbus | Columbus, OH | Food Manufacturing311211 | 20–99 | ◐ LIKELY ACTIVE |
| ANAriel Newark | Heath, OH | Machinery Manufacturing333912 | 100–499 | ◐ LIKELY ACTIVE |
| ALArmaly LLC | London, OH | Fabricated Metal Product Manufacturing332999 | 20–99 | ◐ LIKELY ACTIVE |
| ASAutomotive Supplier | Grove City, OH | Plastics and Rubber Products Manufacturing326199 | 100–499 | ◐ LIKELY ACTIVE |
| AJAWI Johnstown | Johnstown, OH | Fabricated Metal Product Manufacturing332322 | 20–99 | ◐ LIKELY ACTIVE |
| APAxium Packaging : New Albany Plant 1 | New Albany, OH | Plastics and Rubber Products Manufacturing326160 | 100–499 | ◐ LIKELY ACTIVE |
| APAxium Packaging : New Albany Plant 2 | New Albany, OH | Plastics and Rubber Products Manufacturing326160 | 100–499 | ◐ LIKELY ACTIVE |
| BFBaker Family Spirits LLC | Gahanna, OH | Beverage and Tobacco Product Manufacturing312140 | — | ◐ LIKELY ACTIVE |
| BMBanner Metals Group, INC. | Columbus, OH | Transportation Equipment Manufacturing336413 | — | ◐ LIKELY ACTIVE |
| BHBattelle - Hilliard Operations | Hilliard, OH | Transportation Equipment Manufacturing336992 | 20–99 | ◐ LIKELY ACTIVE |
| BHBattelle - Hilliard Operations BCS4 | Hilliard, OH | Transportation Equipment Manufacturing336992 | 1–19 | ◐ LIKELY ACTIVE |
| BCBedrock Creative Innovations LLC | Westerville, OH | Miscellaneous Manufacturing339113 | — | ◐ LIKELY ACTIVE |
| BPBehr Paint Company Heath MFG | Heath, OH | Chemical Manufacturing325510 | 20–99 | ◐ LIKELY ACTIVE |
| BCBell Columbus | Groveport, OH | Miscellaneous Manufacturing339115 | 100–499 | ◐ LIKELY ACTIVE |
| BWBenchmark Woodworks Co. | Delaware, OH | Furniture and Related Product Manufacturing337212 | 20–99 | ◐ LIKELY ACTIVE |
| BLBeneLeaves Limited | Columbus, OH | Chemical Manufacturing325411 | 20–99 | ◐ LIKELY ACTIVE |
| BDBlack Diamond Distillery | New Straitsville, OH | Beverage and Tobacco Product Manufacturing312140 | — | ◐ LIKELY ACTIVE |
| BBBlu Bird LLC dba Stone Center | Columbus, OH | Nonmetallic Mineral Product Manufacturing327991 | 20–99 | ◐ LIKELY ACTIVE |
| BLBlue Label Packaging Company | Lancaster, OH | Printing and Related Support Activities323111 | 100–499 | ◐ LIKELY ACTIVE |
| BBBobbie Baby, Inc | Heath, OH | Food Manufacturing311514 | 100–499 | ◐ LIKELY ACTIVE |
| BIBoehringer Ingelheim Pharmaceuticals - Columbus, OH | Columbus, OH | Chemical Manufacturing325412 | 20–99 | ◐ LIKELY ACTIVE |
| BCBokes Creek Winery | Raymond, OH | Beverage and Tobacco Product Manufacturing312130 | — | ◐ LIKELY ACTIVE |
| BDBoss Display | Columbus, OH | Furniture and Related Product Manufacturing337127 | 20–99 | ◐ LIKELY ACTIVE |
| BDBostik Dublin | Dublin, OH | Chemical Manufacturing325520 | 20–99 | ◐ LIKELY ACTIVE |
| B3Brewery 33 Hocking Hills LLC | Logan, OH | Beverage and Tobacco Product Manufacturing312130 | — | ◐ LIKELY ACTIVE |
| B3Brewery 33 Hocking Hills LLC | Logan, OH | Beverage and Tobacco Product Manufacturing312130 | — | ◐ LIKELY ACTIVE |