| KGKlogo Group, INC | Sioux Falls, SD | Apparel Manufacturing315990 | — | ◐ LIKELY ACTIVE |
| LWLaughing Waters, INC. | Hartford, SD | Beverage and Tobacco Product Manufacturing312130 | — | ◐ LIKELY ACTIVE |
| LMLee's Meats & Sausage, Inc. | Tea, SD | Food Manufacturing311612 | — | ◐ LIKELY ACTIVE |
| LLLineage Logistics, LLC | Luverne, MN | — | — | ◐ LIKELY ACTIVE |
| LSLubricants Sioux Falls | Sioux Falls, SD | Petroleum and Coal Products Manufacturing324191 | 1–19 | ◐ LIKELY ACTIVE |
| NLNevr Looz LLC | Sioux Falls, SD | Miscellaneous Manufacturing339920 | — | ◐ LIKELY ACTIVE |
| NVNew Vision Coop-Magnolia | Magnolia, MN | Food Manufacturing311119 | 20–99 | ◐ LIKELY ACTIVE |
| NSNorcraft - Sioux Falls | Sioux Falls, SD | Furniture and Related Product Manufacturing337110 | 100–499 | ◐ LIKELY ACTIVE |
| OFOrion Food Systems, LLC | Sioux Falls, SD | Food Manufacturing311612 | — | ◐ LIKELY ACTIVE |
| PMPanacea Meadery | Sioux Falls, SD | Beverage and Tobacco Product Manufacturing312130 | — | ◐ LIKELY ACTIVE |
| PMPFG Manufacturing OLM Food Systems Sioux Falls - 4040 | Sioux Falls, SD | Machinery Manufacturing333241 | 100–499 | ◐ LIKELY ACTIVE |
| PMP & M Steel | Sioux Falls, SD | Fabricated Metal Product Manufacturing332312 | 20–99 | ◐ LIKELY ACTIVE |
| PBPOET Bioprocessing- Chancellor | Chancellor, SD | Chemical Manufacturing325193 | 20–99 | ◐ LIKELY ACTIVE |
| PDProSmiles Dental Studio Sioux Falls | Sioux Falls, SD | Miscellaneous Manufacturing339116 | 1–19 | ◐ LIKELY ACTIVE |
| PRProsperous Robotics Group, INC. | Sioux Falls, SD | Miscellaneous Manufacturing339113 | — | ◐ LIKELY ACTIVE |
| PDPro-Tec Division | Lennox, SD | Machinery Manufacturing333111 | 20–99 | ◐ LIKELY ACTIVE |
| RHRaven HQ | Sioux Falls, SD | Computer and Electronic Product Manufacturing334513 | 100–499 | ◐ LIKELY ACTIVE |
| RDRegional Dental Lab, LLC | Sioux Falls, SD | Miscellaneous Manufacturing339116 | — | ◐ LIKELY ACTIVE |
| RCRenner Corner Jerky | Renner, SD | Food Manufacturing311612 | — | ◐ LIKELY ACTIVE |
| SLSenproco, LLC | Sioux Falls, SD | Chemical Manufacturing325611 | 20–99 | ◐ LIKELY ACTIVE |
| SFSioux Falls Admin Office | Sioux Falls, SD | Food Manufacturing311119 | 20–99 | ◐ LIKELY ACTIVE |
| SFSioux Falls Kitchen & Bath | Sioux Falls, SD | Furniture and Related Product Manufacturing337110 | 100–499 | ◐ LIKELY ACTIVE |
| SFSioux Falls Location | Sioux Falls, SD | Printing and Related Support Activities323111 | 20–99 | ◐ LIKELY ACTIVE |
| SFSioux Falls Regional Bakery - Main Store | Sioux Falls, SD | Food Manufacturing311812 | 20–99 | ◐ LIKELY ACTIVE |
| SSSioux Steel Livestock | Lennox, SD | Machinery Manufacturing333111 | 20–99 | ◐ LIKELY ACTIVE |
| SFSmithfield Foods | Sioux Falls, SD | Food Manufacturing311611 | 500+ | ◐ LIKELY ACTIVE |
SONIFI Health, Inc. Sioux Falls Office | Sioux Falls, SD | Computer and Electronic Product Manufacturing334220 | 20–99 | ◐ LIKELY ACTIVE |
| SDSouth Dakota | Brandon, SD | Miscellaneous Manufacturing339930 | 1–19 | ◐ LIKELY ACTIVE |
| SBSpartan - Brandon | Brandon, SD | Transportation Equipment Manufacturing336120 | 100–499 | ◐ LIKELY ACTIVE |
| SWStrawbale Winery | Renner, SD | Beverage and Tobacco Product Manufacturing312130 | — | ◐ LIKELY ACTIVE |
| SCSweetman Construction Company d/b/a Concrete Materials | Sioux Falls, SD | Nonmetallic Mineral Product Manufacturing327320 | 100–499 | ◐ LIKELY ACTIVE |
| TITeamco INC. | Tea, SD | Machinery Manufacturing333120 | 1–19 | ◐ LIKELY ACTIVE |
| TBTerex Bidwell | Canton, SD | Machinery Manufacturing333120 | 20–99 | ◐ LIKELY ACTIVE |
| TGThe Gardens Winery | Alcester, SD | Beverage and Tobacco Product Manufacturing312130 | — | ◐ LIKELY ACTIVE |
| TBThermoBond Buildings | Brandon, SD | Wood Product Manufacturing321992 | 100–499 | ◐ LIKELY ACTIVE |
| TWTucker's Walk Vineyard | Garretson, SD | Beverage and Tobacco Product Manufacturing312130 | — | ◐ LIKELY ACTIVE |
| VGVery Good Meat Company | Hudson, SD | Food Manufacturing311611 | — | ◐ LIKELY ACTIVE |
| VBViaflex - Brandon | Brandon, SD | Plastics and Rubber Products Manufacturing326113 | 1–19 | ◐ LIKELY ACTIVE |
Walman Optical | Sioux Falls, SD | Machinery Manufacturing333249 | 1–19 | ◐ LIKELY ACTIVE |
| WTWheeler Tank Manufacturing, Inc. | Sioux Falls, SD | Fabricated Metal Product Manufacturing332420 | 20–99 | ◐ LIKELY ACTIVE |
| WHWhite Headed Robin Winery | Centerville, SD | Beverage and Tobacco Product Manufacturing312130 | — | ◐ LIKELY ACTIVE |
| WPWilde Prairie Winery | Brandon, SD | Beverage and Tobacco Product Manufacturing312130 | — | ◐ LIKELY ACTIVE |
| WCWin Chill Cold Storage, LLC | Sioux Falls, SD | — | — | ◐ LIKELY ACTIVE |
| SMSunderman Manufacturing Company | Baltic, SD | Machinery Manufacturing333415 | — | ◇ UNVERIFIED |
| 2B2 Brothers Fabrication LLC | Lennox, SD | Fabricated Metal Product Manufacturing332999 | — | ◇ UNVERIFIED |
| AMAgile Manufacturing Group, Inc. | Worthing, SD | Machinery Manufacturing333120 | — | ◇ UNVERIFIED |
| ASAltered Species Ales LLC | Sioux Falls, SD | Beverage and Tobacco Product Manufacturing312120 | — | ◇ UNVERIFIED |
| AIAmerican Ink Properties, LLC | Sioux Falls, SD | Printing and Related Support Activities323113 | — | ◇ UNVERIFIED |
| AGAVPRO Global Holdings LLC | Sioux Falls, SD | Computer and Electronic Product Manufacturing334419 | — | ◇ UNVERIFIED |
| BMBackdraft Manufacturing, LLC | Luverne, MN | Fabricated Metal Product Manufacturing332710 | — | ◇ UNVERIFIED |