| CDCoyne & Delany Company | Charlottesville | VA | Fabricated Metal Product Manufacturing332913 | 1-19 | ◇ UNVERIFIED |
| ACAllied - Charlottesville | Charlottesville | VA | Nonmetallic Mineral Product Manufacturing327320 | — | ◇ UNVERIFIED |
| IIIvy Industries INC | Charlottesville | VA | Machinery Manufacturing333310 | — | ◇ UNVERIFIED |
| RRRockingham Redi- Mix INC - Charlottesville | Charlottesville | VA | Nonmetallic Mineral Product Manufacturing327320 | — | ◇ UNVERIFIED |
| RQRockydale Quarries - Portable Crushing Plant | Charlottesville | VA | Machinery Manufacturing333120 | — | ◇ UNVERIFIED |
| SLS L Williamson & CO - Shadwell | Charlottesville | VA | Petroleum and Coal Products Manufacturing324121 | — | ◇ UNVERIFIED |
| BPBailey Printing | Charlottesville | VA | Printing and Related Support Activities323110 | 1-19 | ◇ UNVERIFIED |
| BSBiomic Sciences LLC | Charlottesville | VA | Chemical Manufacturing325411 | 20-99 | ◇ UNVERIFIED |
| CBChampion Brewing Company, LLC | Charlottesville | VA | Beverage and Tobacco Product Manufacturing312120 | 20-99 | ◇ UNVERIFIED |
| JTJKM Technologies LLC | Charlottesville | VA | Leather and Allied Product Manufacturing316210 | 1-19 | ◇ UNVERIFIED |
| VSVaneri Studio | Charlottesville | VA | Furniture and Related Product Manufacturing337122 | 1-19 | ◇ UNVERIFIED |
| AWAker Wade Power Technologies | Charlottesville | VA | Transportation Equipment Manufacturing336322 | — | ◇ UNVERIFIED |
| AEAlbemarle Edibles | Charlottesville | VA | Food Manufacturing311919 | — | ◇ UNVERIFIED |
| ASAssociated Steel Products INC | Charlottesville | VA | Primary Metal Manufacturing331111 | — | ◇ UNVERIFIED |
| BSBurruss Signs INC | Charlottesville | VA | Chemical Manufacturing325510 | — | ◇ UNVERIFIED |
| BVBurton Ventures, LLC | Charlottesville | VA | Beverage and Tobacco Product Manufacturing312120 | — | ◇ UNVERIFIED |
| COCrown Orchard Co., LP LLP | Charlottesville | VA | Food Manufacturing311813 | — | ◇ UNVERIFIED |
| CLCustomink LLC | Charlottesville | VA | Printing and Related Support Activities323113 | — | ◇ UNVERIFIED |
| DHDefense Holdings INC | Charlottesville | VA | Electrical Equipment, Appliance & Component Manufacturing335312 | — | ◇ UNVERIFIED |
| ETEric Trump Winery LLC. | Charlottesville | VA | Beverage and Tobacco Product Manufacturing312130 | — | ◇ UNVERIFIED |
| JMJohn M Anderson Construction | Charlottesville | VA | Wood Product Manufacturing321918 | — | ◇ UNVERIFIED |
| KWKidde Walter Portable Equipment | Charlottesville | VA | Textile Product Mills314999 | — | ◇ UNVERIFIED |
| KEKluge Estate Winery & Vinyard | Charlottesville | VA | Beverage and Tobacco Product Manufacturing312130 | — | ◇ UNVERIFIED |
| NANo Acquisition Corp, Dba National Optronics | Charlottesville | VA | Machinery Manufacturing333249 | — | ◇ UNVERIFIED |
| NBNothing Bundt Cakes | Charlottesville | VA | Food Manufacturing311811 | — | ◇ UNVERIFIED |
| NLNplainvue LLC Trading As First Colony Winery | Charlottesville | VA | Beverage and Tobacco Product Manufacturing312130 | — | ◇ UNVERIFIED |
| PBPepsi-cola Bottling CO. of Central Va | Charlottesville | VA | Beverage and Tobacco Product Manufacturing312111 | — | ◇ UNVERIFIED |
| RBRenee's Boutique LLC | Charlottesville | VA | Apparel Manufacturing315990 | — | ◇ UNVERIFIED |
 Silver Ring Splint Company | Charlottesville | VA | Fabricated Metal Product Manufacturing332999 | — | ◇ UNVERIFIED |
 Southern States Cooperative, INC. | Charlottesville | VA | Food Manufacturing311119 | — | ◇ UNVERIFIED |
| ZTZebrz Test Facility | Charlottesville | VA | Paper Manufacturing322121 | — | ◇ UNVERIFIED |
