| DND.r. Nickelson and Company, INC. | Lake City | FL | Wood Product Manufacturing321918 | 1-19 | ◐ LIKELY ACTIVE |
| GIGlobal Innovation, LLC | Lake City | FL | Plastics and Rubber Products Manufacturing326199 | 20-99 | ◐ LIKELY ACTIVE |
| ACAnderson Columbia INC | Lake City | FL | Petroleum and Coal Products Manufacturing324121 | — | ◐ LIKELY ACTIVE |
| LOLand O'lakes Purina Feed LLC - Lake City | Lake City | FL | Food Manufacturing311119 | 20-99 | ◐ LIKELY ACTIVE |
| HPHunter Panels LLC | Lake City | FL | Plastics and Rubber Products Manufacturing326140 | — | ◐ LIKELY ACTIVE |
| SMSigncraft & More, INC | Lake City | FL | Miscellaneous Manufacturing339950 | — | ◐ LIKELY ACTIVE |
| TLTownhomes, LLC | Lake City | FL | Wood Product Manufacturing321991 | 100-499 | ◐ LIKELY ACTIVE |
| AMA Materials Group INC. | Lake City | FL | Nonmetallic Mineral Product Manufacturing327320 | — | ◐ LIKELY ACTIVE |
| CSC-bea Stainless Products INC. | Lake City | FL | Machinery Manufacturing333415 | — | ◐ LIKELY ACTIVE |
| CHChampion Home Builders, INC. | Lake City | FL | Wood Product Manufacturing321991 | 100-499 | ◐ LIKELY ACTIVE |
| FCFurst-McNess Company (Lake City) | Lake City | FL | Food Manufacturing311119 | 20-99 | ◐ LIKELY ACTIVE |
| BFBuilders Firstsource - Florida, LLC | Lake City | FL | Wood Product Manufacturing321213 | — | ◐ LIKELY ACTIVE |
| CRColumbia Ready Mix - Lake City | Lake City | FL | Nonmetallic Mineral Product Manufacturing327320 | — | ◐ LIKELY ACTIVE |
| ITIdaho Timber of Florida | Lake City | FL | Wood Product Manufacturing321912 | 20-99 | ◐ LIKELY ACTIVE |
| MMM & M Rehab INC | Lake City | FL | Miscellaneous Manufacturing339113 | — | ◐ LIKELY ACTIVE |
| NBNettles Beef Processing Inc. | Lake City | FL | Food Manufacturing311611 | — | ◐ LIKELY ACTIVE |
| RMRobbins Manufacturing Lake City | Lake City | FL | Wood Product Manufacturing321114 | 20-99 | ◐ LIKELY ACTIVE |
| USUnited States Cold Storage | Lake City | FL | — | — | ◐ LIKELY ACTIVE |
| BCBcgneeds Company | Lake City | FL | Transportation Equipment Manufacturing336412 | — | ◐ LIKELY ACTIVE |
| CECity Electric Supply Company | Lake City | FL | Electrical Equipment, Appliance & Component Manufacturing335132 | — | ◐ LIKELY ACTIVE |