| APAdvertisers Press INC | Middleton | WI | Printing and Related Support Activities323111 | — | ◇ UNVERIFIED |
| FPFristam Pumps INC | Middleton | WI | Machinery Manufacturing333911 | — | ◇ UNVERIFIED |
| LMLucigen Middleton Wi | Middleton | WI | Chemical Manufacturing325414 | — | ◇ UNVERIFIED |
| SSSierra Space | Middleton | WI | Transportation Equipment Manufacturing336415 | — | ◇ UNVERIFIED |
| DDDeath's Door Distillery, LLC | Middleton | WI | Beverage and Tobacco Product Manufacturing312140 | — | ◇ UNVERIFIED |
| APAg Precision Formulators | Middleton | WI | Food Manufacturing311999 | — | ◇ UNVERIFIED |
| APAstro Pak LLC | Middleton | WI | Fabricated Metal Product Manufacturing332813 | — | ◇ UNVERIFIED |
| ACAutomated Components, INC. | Middleton | WI | Computer and Electronic Product Manufacturing334512 | — | ◇ UNVERIFIED |
| BBBloom Bindery, LLC | Middleton | WI | Food Manufacturing311811 | — | ◇ UNVERIFIED |
| BIBruker INC | Middleton | WI | Computer and Electronic Product Manufacturing334516 | — | ◇ UNVERIFIED |
| BNBruker Nano, INC. | Middleton | WI | Computer and Electronic Product Manufacturing334516 | — | ◇ UNVERIFIED |
| DLDrs LTD | Middleton | WI | Petroleum and Coal Products Manufacturing324121 | — | ◇ UNVERIFIED |
| ELEpac LLC | Middleton | WI | Printing and Related Support Activities323111 | — | ◇ UNVERIFIED |
| LILycon INC - Middleton - University Ave | Middleton | WI | Nonmetallic Mineral Product Manufacturing327320 | — | ◇ UNVERIFIED |
| MFMiddleton Farmers Cooperative Company | Middleton | WI | Food Manufacturing311119 | — | ◇ UNVERIFIED |
| MMMiddleton Machining & Welding, INC. | Middleton | WI | Fabricated Metal Product Manufacturing332710 | — | ◇ UNVERIFIED |
| MBMiggy's Bakes, LLC | Middleton | WI | Food Manufacturing311811 | — | ◇ UNVERIFIED |
| NINastar, INC | Middleton | WI | Printing and Related Support Activities323119 | — | ◇ UNVERIFIED |
| PTPhoenix Technique LLC | Middleton | WI | Transportation Equipment Manufacturing336310 | — | ◇ UNVERIFIED |
| PMPrefinished Millwork CORP | Middleton | WI | Wood Product Manufacturing321910 | — | ◇ UNVERIFIED |
| SSSierra Space | Middleton | WI | Transportation Equipment Manufacturing336415 | — | ◇ UNVERIFIED |
| VCViking Cues, LLC | Middleton | WI | Miscellaneous Manufacturing339920 | — | ◇ UNVERIFIED |
