Daktronics, INC. | Brookings | SD | Miscellaneous Manufacturing339950 | — | ◐ LIKELY ACTIVE |
| EIEnercept INC | Watertown | SD | Wood Product Manufacturing321992 | 20-99 | ◐ LIKELY ACTIVE |
| GPGlobal Polymer Industries INC | Madison | SD | Plastics and Rubber Products Manufacturing326121 | 100-499 | ◐ LIKELY ACTIVE |
| GIGraco INC - Sioux Falls Facility | Sioux Falls | SD | Machinery Manufacturing333914 | 100-499 | ◐ LIKELY ACTIVE |
| HLHmmi, LLC (formerly Horizontal Machining & Manufacturing, Inc.) | Huron | SD | Machinery Manufacturing333999 | 20-99 | ◐ LIKELY ACTIVE |
| LCLaser Cut | Madison | SD | Fabricated Metal Product Manufacturing332813 | 1-19 | ◐ LIKELY ACTIVE |
| LML & M Radiator, INC. | Yankton | SD | Transportation Equipment Manufacturing336211 | 100-499 | ◐ LIKELY ACTIVE |
| NANew Angus, LLC | Aberdeen | SD | Food Manufacturing311611 | — | ◐ LIKELY ACTIVE |
| PBPoet Biorefining Big Stone | Big Stone City | SD | Chemical Manufacturing325193 | 20-99 | ◐ LIKELY ACTIVE |
| RERedfield Energy, LLC | Redfield | SD | Chemical Manufacturing325110 | 20-99 | ◐ LIKELY ACTIVE |
| SIScotchman Industries INC | Philip | SD | Machinery Manufacturing333512 | 20-99 | ◐ LIKELY ACTIVE |
| SWShowplace Wood Products | Harrisburg | SD | Furniture and Related Product Manufacturing337110 | 500+ | ◐ LIKELY ACTIVE |
| SDSouth Dakota Partners, INC. | Clear Lake | SD | Computer and Electronic Product Manufacturing334510 | 20-99 | ◐ LIKELY ACTIVE |
| SDSouth Dakota Soybean Processors | Volga | SD | Food Manufacturing311222 | 100-499 | ◐ LIKELY ACTIVE |
Trail-eze INC. | Mitchell | SD | Transportation Equipment Manufacturing336212 | 20-99 | ◐ LIKELY ACTIVE |
Trail King Industries, INC | Mitchell | SD | Transportation Equipment Manufacturing336212 | — | ◐ LIKELY ACTIVE |
| VMVrc Metal Systems LLC | Box Elder | SD | Machinery Manufacturing333519 | 20-99 | ◐ LIKELY ACTIVE |
| CFCustom Fabricators INC. | Watertown | SD | Fabricated Metal Product Manufacturing332999 | 100-499 | ◐ LIKELY ACTIVE |
| DTDakota Tube INC | Watertown | SD | Fabricated Metal Product Manufacturing332813 | 100-499 | ◐ LIKELY ACTIVE |
| FSFiberglass Specialties INC | Watertown | SD | Plastics and Rubber Products Manufacturing326199 | 1-19 | ◐ LIKELY ACTIVE |
| HSHfw Solutions | Rapid City | SD | Miscellaneous Manufacturing339999 | 20-99 | ◐ LIKELY ACTIVE |
| MMMasaba Mining Equipment | Vermillion | SD | Machinery Manufacturing333131 | 100-499 | ◐ LIKELY ACTIVE |
| MFMeyerink Farm Service INC. | Platte | SD | Machinery Manufacturing333111 | 20-99 | ◐ LIKELY ACTIVE |
| MMMitchell Manufacturing, LLC | Mitchell | SD | Miscellaneous Manufacturing339920 | 20-99 | ◐ LIKELY ACTIVE |
| OLOmega Liner Company, INC. | Canton | SD | Plastics and Rubber Products Manufacturing326122 | 20-99 | ◐ LIKELY ACTIVE |
| SCSchoenhard Custom Cabinetry | Tea | SD | Furniture and Related Product Manufacturing337110 | 1-19 | ◐ LIKELY ACTIVE |
| SISencore INC. | Sioux Falls | SD | Computer and Electronic Product Manufacturing334290 | 20-99 | ◐ LIKELY ACTIVE |
| WMWheeler Manufacturing Co, INC. | Lemmon | SD | Miscellaneous Manufacturing339911 | 20-99 | ◐ LIKELY ACTIVE |
| AMAberdeen Machine Tool Inc. | Aberdeen | SD | Machinery Manufacturing333517 | 20-99 | ◐ LIKELY ACTIVE |
| ANAcco Nutting | Watertown | SD | Machinery Manufacturing333924 | 20-99 | ◐ LIKELY ACTIVE |
| APAg Processing, Inc.- Aberdeen Soybean Processing Plant | Aberdeen | SD | Food Manufacturing311222 | — | ◐ LIKELY ACTIVE |
| ANAgriCare Nutrition | Sioux Falls | SD | Food Manufacturing311119 | 20-99 | ◐ LIKELY ACTIVE |
Associated Milk Producers, INC | Freeman | SD | Food Manufacturing311514 | — | ◐ LIKELY ACTIVE |
| AHAtlas Hydraulics INC | Brandon | SD | Fabricated Metal Product Manufacturing332912 | 100-499 | ◐ LIKELY ACTIVE |
| BFBenchmark Foam, INC. | Watertown | SD | Chemical Manufacturing325211 | 20-99 | ◐ LIKELY ACTIVE |
Caw Industries, INC. | Rapid City | SD | Chemical Manufacturing325998 | 1-19 | ◐ LIKELY ACTIVE |
| CSCreative Surfaces INC | Sioux Falls | SD | Furniture and Related Product Manufacturing337212 | 100-499 | ◐ LIKELY ACTIVE |
| DPDakota Provisions, LLC | Huron | SD | Food Manufacturing311615 | — | ◐ LIKELY ACTIVE |
| DTDakota Turkey Growers | Huron | SD | Food Manufacturing311615 | — | ◐ LIKELY ACTIVE |
| ESEgger Steel Company | Sioux Falls | SD | Fabricated Metal Product Manufacturing332312 | 20-99 | ◐ LIKELY ACTIVE |
| FDFernson Distilling Co. | Sioux Falls | SD | Beverage and Tobacco Product Manufacturing312140 | 1-19 | ◐ LIKELY ACTIVE |
| FIFimco INC | North Sioux City | SD | Machinery Manufacturing333111 | 100-499 | ◐ LIKELY ACTIVE |
| GDGcc Dacotah | Rapid City | SD | Nonmetallic Mineral Product Manufacturing327310 | — | ◐ LIKELY ACTIVE |
| GPGrand Prairie Foods, Inc. | Sioux Falls | SD | Food Manufacturing311612 | 100-499 | ◐ LIKELY ACTIVE |
| HIHarvard Integrations | Tea | SD | Fabricated Metal Product Manufacturing332311 | 100-499 | ◐ LIKELY ACTIVE |
| HEHydro Extrusion Usa, LLC | Yankton | SD | Primary Metal Manufacturing331314 | — | ◐ LIKELY ACTIVE |
| LLectrus | Tea | SD | Fabricated Metal Product Manufacturing332311 | — | ◐ LIKELY ACTIVE |
| LLnc | Lake Norden | SD | Food Manufacturing311513 | — | ◐ LIKELY ACTIVE |
| MAManitou Americas INC | Yankton | SD | Machinery Manufacturing333120 | — | ◐ LIKELY ACTIVE |
| MMMidwest Manufacturing Dba Dakota Panel | Rapid City | SD | Wood Product Manufacturing321219 | — | ◐ LIKELY ACTIVE |